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期刊论文 39

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扫描探针显微镜 2

DNA计算 1

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产品设计；知识推送系统；增强训练集；多分类神经网络；知识匹配 1

产品设计；知识推送；适用概率匹配；多维情境；个性化 1

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Mercury source zone identification using soil vapor sampling and analysis

David WATSON,Carrie MILLER,Brian LESTER,Kenneth LOWE,George SOUTHWORTH,Mary Anna BOGLE,Liyuan LIANG,Eric PIERCE

《环境科学与工程前沿（英文）》 2015年 第9卷 第4期   页码 596-604 doi: 10.1007/s11783-014-0709-2

摘要： Development and demonstration of reliable measurement techniques that can detect and help quantify the nature and extent of elemental mercury (Hg(0)) in the subsurface are needed to reduce uncertainties in the decision-making process and increase the effectiveness of remedial actions. We conducted field tests at the Y-12 National Security Complex in Oak Ridge, Tennessee, USA, to determine if sampling and analysis of Hg(0) vapors in the shallow subsurface (<0.3 m depth) can be used to as an indicator of the location and extent of Hg(0) releases in the subsurface. We constructed a rigid polyvinyl chloride push probe assembly, which was driven into the ground. Soil gas samples were collected through a sealed inner tube of the assembly and were analyzed immediately in the field with a Lumex and/or Jerome Hg(0) analyzer. Time-series sampling showed that Hg vapor concentrations were fairly stable over time, suggesting that the vapor phase Hg(0) was not being depleted and that sampling results were not sensitive to the soil gas purge volume. Hg(0) vapor data collected at over 200 push probe locations at 3 different release sites correlated very well to areas of known Hg(0) contamination. Vertical profiling of Hg(0) vapor concentrations conducted at two locations provided information on the vertical distribution of Hg(0) contamination in the subsurface. We conclude from our studies that soil gas sampling and analysis can be conducted rapidly and inexpensively at large scales to help identify areas contaminated with Hg(0).

关键词： push probe     spill     characterization     mapping     gas    

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带多局部约束的改进push-sum框架分布式优化及其在智能电网中的应用

徐谦,俞楚天,袁翔,韦梦立,刘洪喆

《信息与电子工程前沿（英文）》 2023年 第24卷 第9期   页码 1253-1260 doi: 10.1631/FITEE.2200596

摘要： 为此，在改进的push-sum框架下，本文设计了新的分布式优化算法，并在强连通图的假设下给出了其严格的收敛分析。最后，仿真结果证明了所提算法的良好性能。

关键词： 分布式优化     非一致约束     改进push-sum框架    

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Chemical probe systems for assessing liquid–liquid mixing efficiencies of reactors

《化学科学与工程前沿（英文）》 2023年 第17卷 第10期   页码 1323-1335 doi: 10.1007/s11705-022-2275-7

摘要： Liquid–liquid mixing, including homogeneous and heterogeneous mixing, widely exists in the chemical industry. How to quantitatively characterize the mixing performance is important for reactor assessment and development. As a convenient and direct method for mixing characterization, the chemical probe method uses some special test reactions to characterize the mixing results. Here, the working principle and selection requirements of this method are introduced, and some common chemical probe systems for homogeneous and heterogeneous mixing processes are reviewed. The characteristics and applications of these systems are illustrated. Finally, the development of the new system is proposed.

关键词： mixing     chemical probe     liquid–liquid     heterogeneous    

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A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 103-111 doi: 10.1007/s11705-021-2064-8

摘要： Uridine diphosphate (UDP)-glucuronosyltransferases (UGTs) are enzymes involved in the biotransformation of important endogenous compounds such as steroids, bile acids, and hormones as well as exogenous substances including drugs, environmental toxicants, and carcinogens. Here, a novel fluorescent probe BDMP was developed based on boron-dipyrromethene (BODIPY) with high sensitivity for the detection of UGT1A8. The glucuronidation of BDMP not only exhibited a red-emission wavelength (λex/λem = 500/580 nm), but also displayed an excellent UGT1A8-dependent fluorescence signal with a good linear relationship with UGT1A8 concentration. Based on this perfect biocompatibility and cell permeability, BDMP was successfully used to image endogenous UGT1A8 in human cancer cell lines (LoVo and HCT15) in real time. In addition, BDMP could also be used to visualize UGT1A8 in tumor tissues. These results suggested that BDMP is a promising molecular tool for the investigation of UGT1A8-mediated physiological function in humans.

关键词： UDP-glucuronosyltransferase 1A8     fluorescent probe     subtype selectivity     fluorescence imaging    

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A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 121-127 doi: 10.1007/s11705-021-2051-0

摘要： Lysine lipoylation plays vital roles in cell metabolism and redox processes. For example, removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle. Despite the important functions of lysine lipoylation, the mechanisms for the addition and removal of this modification remain largely unexplored. Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins. For example, immunoaffinity purification-mass spectrometry is one of such tools, which highly relies on antibody efficiency and purification techniques. Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity. Affinity-based labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins. Herein, we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation (eraser) activity and interaction of lysine lipoylation with the eraser at the same time. We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2. We envision that the probe will help delineate the roles of delipoylation enzyme in biology.

关键词： dual-function     fluorescent probe     labeling     photo-cross-linker     lipoylation modification     eraser     sirtuin    

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A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

《化学科学与工程前沿（英文）》 2023年 第17卷 第1期   页码 24-33 doi: 10.1007/s11705-022-2171-1

摘要： Hydrazine is extremely toxic and causes severe harm to human body. Herein, a novel fluorescent probe 4-oxo-2-styryl-4H-chromen-3-yl thiophene-2-carboxylate (FHT) was synthesized for detecting hydrazine by using natural cinnamaldehyde as starting material. This probe exhibited significantly enhanced fluorescence response towards hydrazine over various common metal ions, anions, and amine compounds. The detection limit of probe FHT for hydrazine was as low as 0.14 μmol·L–1, significantly lower than that of the threshold value of 0.312 μmol·L–1, imposed by the Environmental Protection Agency. Moreover, the proposed probe was able to detect hydrazine within wide pH (5–10) and linear detection ranges (0–110 μmol·L–1). This probe was employed for determining trace hydrazine in different environmental water samples. The probe FHT-loaded filter paper strips were able to conveniently detect hydrazine of low concentration through distinct naked-eye and fluorescent color changes. Importantly, the probe FHT with low cytotoxicity was successfully applied to visualize hydrazine in living Hela cells and zebrafish.

关键词： cinnamaldehyde     3-hydroxychromone derivative     hydrazine     fluorescent probe    

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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 92-102 doi: 10.1007/s11705-021-2063-9

摘要： Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases, such as genetic diseases and cancers. Therefore, the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition. Herein, we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN. The fluorophore was 2-styrene-1H-benzo[d]imidazole, and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA. In this design, MBI-CN conjugates to mitochondrial DNA without causing additional damages. Thus, MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment with mitochondrial DNA damage. Meanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA. Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA, it is a potentially effective detection tool for the real-time monitoring of mitochondrial DNA damage during cell apoptosis and initial assessment of cell apoptosis.

关键词： hydrolysis reaction     mitochondrial DNA damage     in situ hydrophobic environment trigger     fluorescence probe     apoptosis    

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A multi-probe micro-fabrication apparatus based on the friction-induced fabrication method

Zhijiang WU, Chenfei SONG, Jian GUO, Bingjun YU, Linmao QIAN

《机械工程前沿（英文）》 2013年 第8卷 第4期   页码 333-339 doi: 10.1007/s11465-013-0276-4

摘要：

A novel multi-probe micro-fabrication apparatus was developed based on the friction-induced fabrication method. The main parts of the apparatus include actuating device, loading system, and control system. With a motorized linear stage, the maximum fabrication area of 50 mm × 50 mm can be achieved, and the maximum sliding speed of probes can be as high as 10 mm/s. Through locating steel micro balls into indents array, the preparation of multi-probe array can be realized by a simple and low-cost way. The cantilever was designed as a structure of deformable parallelogram with two beams, by which the fabrication force can be precisely controlled. Combining the friction-induced scanning with selective etching in KOH solution, various micro-patterns were fabricated on Si(100) surface without any masks or exposure. As a low-cost and high efficiency fabrication device, the multi-probe micro-fabrication apparatus may encourage the development of friction-induced fabrication method and shed new light on the texture engineering.

关键词： friction-induced fabrication     silicon     surface texture     friction     multi-probe    

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全球计时器暂停闰秒计时

Chris Palmer

《工程（英文）》 2023年 第27卷 第8期   页码 6-7 doi: 10.1016/j.eng.2023.06.003

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Review of self-referenced measurement algorithms: Bridging lateral shearing interferometry and multi-probe

Dede ZHAI, Shanyong CHEN, Ziqiang YIN, Shengyi LI

《机械工程前沿（英文）》 2017年 第12卷 第2期   页码 143-157 doi: 10.1007/s11465-017-0432-3

摘要：

With the development of new materials and ultra-precision processing technology, the sizes of measured objects increase, and the requirements for machining accuracy and surface quality become more exacting. The traditional measurement method based on reference datum is inadequate for measuring a high-precision object when the quality of the reference datum is approximately within the same order as that of the object. Self-referenced measurement techniques provide an effective means when the direct reference-based method cannot satisfy the required measurement or calibration accuracy. This paper discusses the reconstruction algorithms for self-referenced measurement and connects lateral shearing interferometry and multi-probe error separation. In lateral shearing interferometry, the reconstruction algorithms are generally categorized into modal or zonal methods. The multi-probe error separation techniques for straightness measurement are broadly divided into two-point and three-point methods. The common features of the lateral shearing interferometry method and the multi-probe error separation method are identified. We conclude that the reconstruction principle in lateral shearing interferometry is similar to the two-point method in error separation on the condition that no yaw error exists. This similarity may provide a basis or inspiration for the development of both classes of methods.

关键词： self-referenced measurement     lateral shearing interferometry     multi-probe error separation     surface metrology    

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先进天线赋能无线互联

陆贵文, 段宝岩

《工程（英文）》 2022年 第11卷 第4期   页码 1-2 doi: 10.1016/j.eng.2022.03.003

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Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence properties

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

《化学科学与工程前沿（英文）》 2007年 第1卷 第4期   页码 381-384 doi: 10.1007/s11705-007-0069-6

摘要： Four novel 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles that exhibit strong two-photon absorption and enhanced two-photon excited fluorescence were designed and synthesized based on push-core-pull-core-push molecules built from embedding electron-transporting 1,3,4-oxadiazole in aromatic conjugated system through Wittig-Horner reaction. Their chemical structures were determined to show trans-vinylene character according to infrared (IR) and H nuclear magnetic resonance (NMR) spectra. A very effective energy transfer from the excited units to the ?-conjugated bridging unit can enhance the two-photon absorption and two-photon fluorescence.

关键词： 2-arylvinyl     trans-vinylene character     effective     fluorescence     push-core-pull-core-push    

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Near-infrared fluorescent probe for fast track of cyclooxygenase-2 in Golgi apparatus in cancer cells

Bhaskar Gurram, Miao Li, Jiangli Fan, Jingyun Wang, Xiaojun Peng

《化学科学与工程前沿（英文）》 2020年 第14卷 第1期   页码 41-52 doi: 10.1007/s11705-019-1796-1

摘要： Cyclooxygenase-2 (COX-2) has been used as an excellent traceable biomarker, and exists maximally in Golgi apparatus (Cancer cells). Celecoxib (CCB) is a selective inhibitor for COX-2, and has been used as one of non-steroidal anti-inflammatory drug. Herein we report the conjugation of nile blue (NB) with CCB via a six-carbon linkage to form a fluorescence probe NB-C6-CCB for the detection of COX-2. NB-C6-CCB displays strong fluorescence with the emission peak centered at near-infrared wavelength (700 nm) in tumor cells or tumor tissues with high expression of COX-2. Importantly, NB-C6-CCB can discriminate cancer cells (MCF-7) fluorescence intensity from normal ones (COS-7) in the co-culture medium under confocal microscope. Subcellular localization of the NB-C6-CCB preferentially points to the Golgi apparatus and increases the fluorescent intensity. The competitive analysis (with CCB) and Native-PAGE analysis confirmed that NB-C6-CCB shows selective binding affinity towards COX-2 enzyme. Competitive analysis with CCB (flow cytometry assay) revealed the fluorescence intensity fluctuation due to pretreatment of CCB with different concentrations, indicating that the NB-C6-CCB is a precise or sensitive probe for the COX-2. Tumor tissue (depth: 500 µm), organs and mice imaging tests show excellent near-infrared visualization, specific localization and identification of tumors.

关键词： cyclooxygenase-2     nile blue     CCB     Golgi apparatus     NIR imaging    

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Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

null

《医学前沿（英文）》 2018年 第12卷 第1期   页码 23-33 doi: 10.1007/s11684-017-0607-7

摘要：

Two decades have passed since the first bacterial whole-genome sequencing, which provides new opportunity for microbial genome. Consequently, considerable genetic diversity encoded by bacterial genomes and among the strains in the same species has been revealed. In recent years, genome sequencing techniques and bioinformatics have developed rapidly, which has resulted in transformation and expedited the application of strategy and methodology for bacterial genome comparison used in dissection of infectious disease epidemics. Bacterial whole-genome sequencing and bioinformatic computing allow genotyping to satisfy the requirements of epidemiological study in disease control. In this review, we outline the significance and summarize the roles of bacterial genome sequencing in the context of bacterial disease control and prevention. We discuss the applications of bacterial genome sequencing in outbreak detection, source tracing, transmission mode discovery, and new epidemic clone identification. Wide applications of genome sequencing and data sharing in infectious disease surveillance networks will considerably promote outbreak detection and early warning to prevent the dissemination of bacterial diseases.

关键词： genome sequencing     genomic epidemiology     bacteria     surveillance     infectious diseases    

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基于适用概率匹配与多维情境驱动的设计知识推送技术 None

Shu-you ZHANG, Ye GU, Xiao-jian LIU, Jian-rong TAN

《信息与电子工程前沿（英文）》 2018年 第19卷 第2期   页码 235-245 doi: 10.1631/FITEE.1700763

摘要： 为了提高产品智能设计过程中设计知识的使用效率和质量，有必要向设计人员主动推送设计知识。知识推送主要包括知识匹配和匹配结果的合理推送两个方面。针对现有知识匹配通常缺乏智能性和匹配结果推送缺少个性化的问题，提出基于适用概率匹配和多维情境驱动的设计知识推送技术。构建包括设计知识表示向量、设计案例特征向量和映射布尔矩阵等的训练样本集，通过贝叶斯理论计算设计知识适用与不适用于设计内容的概率，即二者之间的匹配度，得到推送知识集。构建等级化设计内容模型对推送知识集进行过滤，通过设计知识、设计上下文、设计内容和设计人员等多维情境驱动，实现个性化的设计知识推送。在数控机床智能设计平台中的知识推送应用，证明了该技术的可行性和正确性。

关键词： 产品设计；知识推送；适用概率匹配；多维情境；个性化    

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标题 作者 时间 类型 操作

Mercury source zone identification using soil vapor sampling and analysis

David WATSON,Carrie MILLER,Brian LESTER,Kenneth LOWE,George SOUTHWORTH,Mary Anna BOGLE,Liyuan LIANG,Eric PIERCE

期刊论文

带多局部约束的改进push-sum框架分布式优化及其在智能电网中的应用

徐谦,俞楚天,袁翔,韦梦立,刘洪喆

期刊论文

Chemical probe systems for assessing liquid–liquid mixing efficiencies of reactors

期刊论文

A highly selective fluorescent probe for real-time imaging of UDP-glucuronosyltransferase 1A8 in living

期刊论文

A dual-function chemical probe for detecting erasers of lysine lipoylation

Yusheng Xie, Jie Zhang, Liu Yang, Qingxin Chen, Quan Hao, Liang Zhang, Hongyan Sun

期刊论文

A novel flavonol-based colorimetric and turn-on fluorescent probe for rapid determination of hydrazine

期刊论文

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNA

期刊论文

A multi-probe micro-fabrication apparatus based on the friction-induced fabrication method

Zhijiang WU, Chenfei SONG, Jian GUO, Bingjun YU, Linmao QIAN

期刊论文

全球计时器暂停闰秒计时

Chris Palmer

期刊论文

Review of self-referenced measurement algorithms: Bridging lateral shearing interferometry and multi-probe

Dede ZHAI, Shanyong CHEN, Ziqiang YIN, Shengyi LI

期刊论文

先进天线赋能无线互联

陆贵文, 段宝岩

期刊论文

Synthesis and characterization of 2,5-bis[4-(2-arylvinyl)phenyl]-1,3,4-oxadiazoles with two-photon fluorescence properties

ZHU Xiaoqin, QIAN Ying, LU Zhifeng

期刊论文

Near-infrared fluorescent probe for fast track of cyclooxygenase-2 in Golgi apparatus in cancer cells

Bhaskar Gurram, Miao Li, Jiangli Fan, Jingyun Wang, Xiaojun Peng

期刊论文

Transforming bacterial disease surveillance and investigation using whole-genome sequence to probe the

null

期刊论文

基于适用概率匹配与多维情境驱动的设计知识推送技术

Shu-you ZHANG, Ye GU, Xiao-jian LIU, Jian-rong TAN

期刊论文